The Giant Mutant Widow Spiders (ジャイアントウィドースパイダー?, lit. Giant Widow Spider) are giant mutated arachnid Jaiantou Idōsupaidākaiju created by TriStar Pictures that first appeared in the Godzilla: The Series episode Web Site.
The Giant Mutant Widow Spiders were first seen in South America, where they attacked a man who ventured into the jungle. H.E.A.T. was then contacted by the Military to investigate. They collected a few small Spiders, and were attacked by many more Spiders. Despite the military's attempt to kill them with pesticides, the Spiders were immune to most toxins. The Queen Mutant Widow Spider then attacked the military base, where it faced Godzilla. The Queen attacked him with her webbing, but Godzilla was able to break out before both monsters retreated. After returning to its nest, the Queen laid more eggs. H.E.A.T. and the military then discovered that a certain type of pollen paralyzes the Spiders and used this against them at the Spiders' nest. They left their pollen payload in the nest, but were attacked by more Spiders and Godzilla was called to rescue them. He held off the Spiders long enough for the bomb to detonate. Godzilla survived the explosion while the Spiders are rendered paralyzed from the pollen's effects.
- The Giant Widow Spider is a tribute to Kumonga, along with monsters from other "giant spider" films such as The Giant Spider Invasion.
- The Giant Widow Spider appeared in promo art for the Monster Wars trilogy, but did not appear in the trilogy.